tRNA cleavage fragments are gaining considerable attention for their biological roles and application as biomarkers, making their analysis in small RNA data highly desirable.
An additional regulatory role is through cleaveage-products, tRNA halves (Angiogenin cleaved) or smaller tRNA fragments (Dicer cleaved). These are highly expressed in exosomes, accumulate in certain diseases (biomarker) and in some cases have been shown to act similar to miRNA together with Ago.
The quantification of tRNA cleavage products can be performed on small-RNA sequencing data and can be the primary purpose of sequencing, or it can be an additional analysis performed on data generated for miRNA quantification.
'Principal Component Analysis (PCA) Plot is used to give you a quick visual overview of the general difference between your samples in tRNA expression.
The Volcano plot is a visual representation of all tRNAs, correlating the change in expression level between the samples. Each dot denotes a specific tRNA. Black dots represent non-significant changes, while red dots display significant values.
In addition to comparisons between groups, you will get fragment mapping on the individual full-length tRNAs, tRNA coverage, and read length to enable quick and easy detection of significant trends.
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