TOTAL RNA SEQUENCING
Total RNA-seq, transcriptome analysis, mRNA-seq, Long non-coding RNA-seq, RNA-seq for quantification & 3’end sequencing
While the genome in an organism is considered to be mostly static over time, changes associated with development, aging and disease is reflected in the transcriptome. Comparative studies applying RNA-seq enables investigation of changes in expression level, isoform usage and regulatory events as circularization.
While each project is unique and consequently the optimal solution varies according to the settings, some elements will be fairly consistent across most experiements.
- 250-300 bp insert cDNA library
- Multiple kit options available
* PolyA-enrichment or cDNA capture is also available, however, we highly recommend using rRNA removal as this provides data on both coding and noncoding RNAs.
** Non-stranded sequencing is available, however, gives you less valuable data and while historical cheaper, the current prices are identical.
PE: Paired-end, SE: Single-end
Publication-grade figures (.TIFF*)
* Other formats are also available if requested
Additional Bioinformatic Analysis for Total RNA sequencing
Pathway analysis (Gene ontology analysis, Pathway enrichment analysis
Network analysis, including Condition specific expressed analysis, Gene Co-expression Network
Analysis, Time series analysis
CircRNA detection and circRNA differential expression analysis
Alternative splicing/isoform usage analysis/Fusion gene analysis
LncRNA detection and function prediction /LncRNA Family prediction and classification
SNP and Indel detection
Protein-Protein Interaction (PPI) analysis
Comparison with online available data (Data-mining)
NGS service provider with a focus on customer needs. We deliver flexible solutions to your specific demands.
Contact us for a non-binding discussion about your next project.
(+45) 28 72 71 07
CVR: 39 73 23 43