To prepare your material for deep-sequencing, three essential steps are required in the laboratory. At omiics, we have the facilities and experience to conduct all three for you.
Today, a wide selection of library preparation kits available and the best choice depends on the input quality and quantity, as well as the type of end-analysis needed.
Make it easy for yourself - Let omiics choose the optimal reagents for your setup.
Deplete the many or enrich the few?
Ribosomal RNA (rRNA) comprises a significant proportion (~90% or more) of all the RNA in total RNA samples. While the rRNA will not directly cause harm to the result, removing rRNA or enrichment of mRNA prior to generating libraries provides benefits by lowering sequencing costs and improving mapping statistics.
Unique molecular identifyer (UMI)
A speciality of omiics is sequencing ultra-low input samples down to as low as 250pg RNA.
At ultra-low RNA concentration, the sample becomes more sensitive to PCR-associated bias. By including an additisonal molecular tag during library preparation, the unique molecular identifier, UMI, enables you to get high-resolution reads, to facilitate accurate detection of true variants.